Coding
Part:BBa_K3032013:Design
Designed by: Denis Baronas Group: iGEM19_Vilnius-Lithuania (2019-10-12)
CarH repressor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was codon optimized for E. coli.
Source
CarH protein sequence was taken from https://www.uniprot.org/uniprot/Q53W62 (Thermus thermophilus). DNA sequence was codon optimized for E. coli.